WebCell lines C13 and OVCAR5 were cultured and transfected with CTSL-sh1 (TGCCTCAGCTACTCTAACAT) sh2 (TGCCTCAGCTACTCTAACATT) and with nontargeted control shRNA as control with Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) as per manufacturer protocol. Stable clones were selected by puromycin as reported earlier [20]. … WebHuman ovarian cancer cell lines HeyA8-MDR from MD Anderson (TX, USA), OVCAR5, OVCAR7, C13, OV2008 and Hek293T cells from ATCC (VA, USA) were grown in RPMI media (Gibco, Waltham, MA, USA) supplemented with 10% fetal bovine serum (R&D Systems, Minneapolis, MN, USA) and 1% penicillin–streptomycin 5% CO2–95% air humidified …
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WebDarko Ovcar posted images on LinkedIn WebTable S2 . Cell lines Media Supplements OVCAR5 / OVCAR8/ PEO1/ PEO4/ OVCAR4 / OVCAR10/ OV202 RPMI-1640 10% FBS and 1% Pen/Strep OVCAR7 DMEM (4.5 g/l glucose) 10% FBS, 1% Pen/Strep and insulin how to clean and prep bok choy
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WebMAD2 in the paclitaxel resistant UPN251 and OVCAR7 cell lines. While, all 4 paclitaxel resistant EOC cell lines express higher levels of miR-433 than the paclitaxel sensitive A2780 cells, only ovca432 and ovca433 demonstrated down-regulation of both HDAC6 and MAD2. Paclitaxel binds to b-tubulin and WebDec 1, 2024 · Results: Median age was 63 years (range, 38-85), 37% were White, 58% were Black and 5% were Asian. GA were identified in 94% (81/86) of these patients. TP 53 mutations which is characteristic of serous cancer were present in 80 of 86 patients (93%). WebOVCAR-3 xenograft model OVCAR-3 cells are known to express high levels of the estrogen receptor (ER) and progesterone receptor (PR), and they are sensitive to hormones such as … how to clean and polish wood floors