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WebMar 29, 2012 · Asparaginase inclusion bodies were completely solubilized at 2–3 M urea concentration and could be refolded into active protein, whereas 7 M urea was required for complete solubilization of hGH inclusion bodies. ... Panda AK (2003) Bioprocessing of therapeutic proteins from the inclusion bodies of Escherichia coli. Adv Biochem … WebAbstract High-level expression of recombinant proteins in Escherichia coli often results in accumulation of protein molecules into aggregates known as inclusion bodies (IBs). Isolation of properly folded, bioactive protein from IBs is a cumbersome task and most of the times results in poor recovery. small conservatory interior design ideas
Protein recovery from inclusion bodies of Escherichia coli using …
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