High-density oligonucleotide arrays

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August undefined, 2024

Web16 de mar. de 2009 · The high-density oligonucleotide array analysis was done using an Illumina Infinium whole-genome genotyping 550K Beadchip (Illumina). Genomic DNA (750 ng) from the tumor sample was processed using reagents and protocols supplied by Illumina in The Children's Hospital of Philadelphia Center for Applied Genomics . WebWe have developed an approach in which sequence information is used directly to design high-density, two-dimensional rays of synthetic oligonucleotides. The GeneChipe …

Functional Genomics: High-Density Oligonucleotide Arrays

Web24 de mai. de 1994 · These probe arrays, or DNA chips, can then be applied to parallel DNA hybridization analysis, directly yielding sequence information. In a preliminary … Web1 de jan. de 1996 · Refereed paperHigh-density oligonucleotide arrays. We are attempting to produce high-density arrays of surface-bound oligonucleotides for hybridization experiments. Our approach is to mechanically miniaturize conventional DNA synthesis chemistry. Ink-jet printer heads are used to deliver small drops of reagents to a … sharon grove ky weather radar

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Web1 de jan. de 2002 · The leading arrays in the category of high-density oligonucleotide arrays are manufactured by Affymetrix and utilize the combinatorial synthesis principle. The arrays are designed by using a light-directed chemical synthesis process that creates a series of photolithographic masks to define chip exposure sites, followed by specific … Web1 de nov. de 1996 · High-density arrays of oligonucleotide probes are proving to be powerful new tools for large-scale DNA and RNA sequence analysis. A method for constructing these arrays, using light-directed DNA synthesis with photoactivatable monomers, can currently achieve densities on the order of 10 6 sequences/cm 2.One of … Web1 de jan. de 1997 · Oligonucleotide arrays are also used for DNA sequencing and genotyping (Gingeras et al., 1998), which is a promising application of the high-density oligonucleotide hybridization platform. ... population threshold biology

DNA microarrays and their applications in medical microbiology

High-density oligonucleotide arrays

Detection of heterozygous mutations in BRCA1 using high density ...

http://scgc.genetics.ucla.edu/sites/default/files/publications/2006%20Advancement%20in%20characterization%20of%20genomic%20alterations%20for%20improved%20diagnosis%20treatment%20and%20prognostics%20in%20cancer.pdf WebThis work presents a low-cost method to produce compact arrays using microporous materials and reagent jetting, particularly useful for producing identical arrays from pre …

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Webnucleotide polymorphism array, spectral karyotyping, tiling array Most human cancers are characterized by genetic instabilities. These instabilities manifest themselves as a series of genetic alterations, including discrete mutations and chromosomal aberrations. With the human genome deciphered, high-throughput Web1 de jan. de 2002 · High-density DNA probe arrays provide a highly parallel approach to nucleic acid sequence analysis that is transforming gene-based biomedical research. Photolithographic DNA synthesis has enabled the large-scale production of GeneChip probe arrays containing hundreds of thousands of oligonucleotide sequences on a glass …

Web1 de dez. de 1996 · We have developed an approach that is based on hybridization to small, high-density arrays containing tens of thousands of synthetic oligonucleotides. The … WebAlthough oligonucleotide probes are often used in "spotted" microarrays, the term "oligonucleotide array" most often refers to a specific technique of manufacturing. …

Web22 de jan. de 2003 · Motivation: When running experiments that involve multiple high density oligonucleotide arrays, it is important to remove sources of variation between arrays of non-biological origin. Normalization is a process for reducing this variation. Web1 de dez. de 1996 · Hacia, J., Brody, L., Chee, M. et al. Detection of heterozygous mutations in BRCA1 using high density oligonucleotide arrays and two–colour fluorescence analysis. Nat Genet 14 , 441–447 (1996 ...

WebFigure 2: Gene expression monitoring with oligonucleotide arrays. a, A single 1.28×1.28 cm array containing probe sets for approximately 40,000 human genes and ESTs. This array contains features ...

WebHepatic NK cells are more cytotoxic than blood NK cells against tumor cells. To understand the basis of this difference in cytotoxicity we analyzed RNA derived from freshly isolated … sharon grove ky weatherWebWe have developed an approach in which sequence information is used directly to design high–density, two–dimensional arrays of synthetic oligonucleotides. The GeneChip® probe arrays are made using spatially patterned, light–directed combinatorial chemical synthesis, and contain up to hundreds of thousands of different oligonucleotides on a small glass … population tianjin chinaWeb18 de mai. de 2007 · In spotted arrays, complementary DNA or long oligonucleotide probes are printed onto the substrate. The two messenger RNA samples of interest are reverse transcribed into complementary DNA, labelled with a different fluorescent dye (green cyanine 3 or red cyanine 5) and mixed together, before being washed over the slide and … population thurston county waWebHigh-density DNA probe arrays provide a highly parallel approach to nucleic acid sequence analysis that is transforming gene-based biomedical research. … sharon grove ladwpWeb1 de jan. de 2002 · The leading arrays in the category of high-density oligonucleotide arrays are manufactured by Affymetrix and utilize the combinatorial synthesis principle. 9 The arrays are designed by using a light-directed chemical synthesis process that creates a series of photolithographic masks to define chip exposure sites, followed by specific … sharon gryfe cbcWebOn high-density oligonucleotide microarrays, DNA oligonucleotides are synthesized directly on the microarray using ultraviolet masks and photoactivated chemistry. 30 … population tiffin ohioWebIn this paper we report exploratory analyses of high-density oligonucleotide array data from the Affymetrix GeneChip system with the objective of improving upon … sharon grover