Biotinylated anti his tag antibody
WebPrimary Antibodies Rabbit anti-6-His Tag Antibody Biotinylated Bethyl Laboratories® Catalog # A190-114B Citations (1) Target: 6-His Tag Application: ELISA , WB Host: Rabbit Clonality: Polyclonal Format: Whole IgG Isotype: IgG Conjugate: Biotin Purity: Antigen Affinity Purified 0.1 mg A190-114B $288.00 Qty: Bulk Request Product Details Applications WebPVDF membrane was probed with 0.2 µg/mL of Mouse Anti-His Tag Recombinant Monoclonal Antibody (Catalog # MAB050R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018 ). Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under …
Biotinylated anti his tag antibody
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Web1 mg/ml. Specificity. Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: … WebA DNA sequence encoding the Recombinant Monkeypox Virus (MPXV) B6R Protein was expressed with a polyhistidine tag at the C-terminus. The purified protein was …
WebKey features and details. Biotin Rabbit polyclonal to 6X His tag®. Suitable for: Flow Cyt, WB, ELISA. Reacts with: Species independent. Conjugation: Biotin. Isotype: IgG. WebHis-tags. Molecular Weight: 0.2–1.6 kDa. 6x-His tag is 0.8 kDa. Size: 2–10 histidine residues Tag location: C- or N- terminals, or internal. Affinity Resin: transition metal ions, usually Ni 2+ Applications: protein purification Strengths: Small size so lower possibility of affecting fusion protein functionality. Doesn’t cause inclusion bodies. Limitations: Can …
WebAll lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution Lane 1 : 6x His-tagged protein expressed in BL21 (pET15b) at 1 µg Lane 2 : 6x His-tagged protein … WebSPR sensor chips. Biacore™ sensor chips enable you study molecular interactions in real time — from small organic molecules to viruses. The sturdier vertical slab gel unit was designed with durability and cost in mind. Injection-moulded of tough, impact-resistant plastics, it will withstand the rigours of daily lab use and teaching labs for ...
WebBiotin-labeled anti-His-tag antibodies are used to indirectly label proteins tagged with 6XHis. This can be advantageous when working with partially purified protein, or in …
WebBiotinylated Anti-His Tag Antibody(AY63), Fab is a Fab fragment of a mouse monoclonal antibody recombinantly expressed from human 293 cells (HEK293). The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with a poly-his tagged protein. did michigan basketball winWebBiotinylated-His peptide, to be used as a probe, assay control, or QC control in Alpha (AlphaLISA®, AlphaScreen®, or AlphaPlex™) no-wash assays. This product contains 0.5 mL of biotinylated-His at 5 µM. This … did michigan beat ohio state this yearWebadjective. bio· tin· y· lat· ed ˌbī-ə-ˈti-nə-ˌlā-təd. : combined with biotin. The PCR reaction uses biotinylated primers to define a sequence of approximately 207 nucleotides within … did michigan beat michigan stateWebIn assay 2, MukB was immobilized by using a His 6 -tagged variant of MukB and biotinylated anti-His 6 -antibody on the PEG surface. Article Snippet: Afterwards, 2 μg/ml Biotinylated Anti-His Antibody (Penta-His Biotin Conjugate; Qiagen ; No. 34440) in T50 buffer was introduced into flow cells at 50 μl/min, then wash immediately with 300ul T50 ... did michigan basketball win tonightWebBiotinylation. In biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. Biotinylation is rapid, specific and is unlikely to disturb the natural function of the molecule due to the small size of biotin (MW = 244.31 g/mol). Biotin binds to streptavidin and avidin with an extremely ... did michigan end daylight savings timeWebAnti-His Tag Antibody [RM146] (Biotin) Description Recombinant rabbit monoclonal [RM146] antibody to His Tag (Biotin). Specificity This antibody recognizes recombinant proteins containing the 6 x His Tag or 10 x His Tag, fused to either the amino or carboxy terminus. Applications WB, IP, ICC, Flow Cytometry, IHC Dilutions did michigan beat rutgers yesterdayWebThis antibody, which contains a mouse IgG2a isoform, was produced as a recombinant protein expressed in HEK293-derived cells using an engineered construct based on a … did michigan car insurance go up